Strategies and Challenges in Measuring Protein Abundance Using Stable Isotope Labeling and Tandem Mass Spectrometry
نویسندگان
چکیده
منابع مشابه
UNiquant, a program for quantitative proteomics analysis using stable isotope labeling.
Stable isotope labeling (SIL) methods coupled with nanoscale liquid chromatography and high resolution tandem mass spectrometry are increasingly useful for elucidation of the proteome-wide differences between multiple biological samples. Development of more effective programs for the sensitive identification of peptide pairs and accurate measurement of the relative peptide/protein abundance are...
متن کاملAnalysis of the Membrane Proteome of Ciprofloxacin-Resistant Macrophages by Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)
Overexpression of multidrug transporters is a well-established mechanism of resistance to chemotherapy, but other changes may be co-selected upon exposure to drugs that contribute to resistance. Using a model of J774 macrophages made resistant to the fluoroquinolone antibiotic ciprofloxacin and comparing it with the wild-type parent cell line, we performed a quantitative proteomic analysis usin...
متن کاملStable Isotope Labeling of Mammals (SILAM).
INTRODUCTIONA general approach in quantitative mass spectrometry is to mix a protein sample containing only natural-abundance isotopes with an identical protein sample containing proteins labeled with heavy stable isotopes (e.g., (2)H, (13)C, (15)N, or (18)O). Introduction of stable isotope labels into proteins alters their molecular weight and such changes can be observed on the mass spectrome...
متن کاملQuantitative Map of Proteome Dynamics during Neuronal Differentiation
Neuronal differentiation is a multistep process that shapes and re-shapes neurons by progressing through several typical stages, including axon outgrowth, dendritogenesis, and synapse formation. To systematically profile proteome dynamics throughout neuronal differentiation, we took cultured rat hippocampal neurons at different developmental stages and monitored changes in protein abundance usi...
متن کاملA New Enhanced, Rapid and Precise Sample Preparation Protocol for Label-free Protein Quantification
Tandem mass spectrometry (MS/MS) in combination with nanoflow liquid chromatography (LC) has become the analytical technique of choice for global relative quantification assays of complex protein samples [1]. Methodologies have been developed to enable the comparison of protein expression levels across multiple samples, which may be broadly classified as either label-free, or those incorporatin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2017